The Bordet Tumour Bank (BTB) offers to cancer researchers a broad range of high quality tissue samples and corresponding data, collected following stringent standardised procedures and ethical guidelines complying with national legislation and international guidelines. BTB contains fresh-frozen/ FFPE tumour and normal adjacent tissue and peripheral blood samples collected from more than 9000 patients. The clinical and non-clinical sample characteristics and patient consent are managed by a relational database. The BTB has allowed Belgian scientists and research investigators, to advance research programs in genetics and cell biology. BTB received ISO-9001:2000 Quality Certification in 2012.